2B95

Solution NMR structure of protein dynein light chain 2A, cytoplasmic; Northeast structural genomics consortium TARGET HR2106


SOLUTION NMR
NMR Experiment
ExperimentTypeSample ContentsSolventIonic StrengthpHPressureTemperature (K)Spectrometer
13D_13C-separated_NOESY1.3mM DYNEIN LIGHT CHAIN 2A, U-15N, 13C, 50 mM MES, 95% H2O, 5% D2O, 0.02% NaN3, pH 6.095% H2O/5% D2O6.0ambient298
23D_15N-separated_NOESY1.3mM DYNEIN LIGHT CHAIN 2A, U-15N, 13C, 50 mM MES, 95% H2O, 5% D2O, 0.02% NaN3, pH 6.095% H2O/5% D2O6.0ambient298
33D_15N_13C_filter-edit simultaneous NOESY1.3mM DYNEIN LIGHT CHAIN 2A, U-15N, 13C, 50 mM MES, 95% H2O, 5% D2O, 0.02% NaN3, pH 6.095% H2O/5% D2O6.0ambient298
4RD 3D HABCABCONHN1.3mM DYNEIN LIGHT CHAIN 2A, U-15N, 13C, 50 mM MES, 95% H2O, 5% D2O, 0.02% NaN3, pH 6.095% H2O/5% D2O6.0ambient298
5GFT (4,3) HNCABCA1.3mM DYNEIN LIGHT CHAIN 2A, U-15N, 13C, 50 mM MES, 95% H2O, 5% D2O, 0.02% NaN3, pH 6.095% H2O/5% D2O6.0ambient298
6GFT (4,3) CABCACONHN1.3mM DYNEIN LIGHT CHAIN 2A, U-15N, 13C, 50 mM MES, 95% H2O, 5% D2O, 0.02% NaN3, pH 6.095% H2O/5% D2O6.0ambient298
NMR Spectrometer Information
SpectrometerManufacturerModelField Strength
1VarianINOVA750
2VarianINOVA600
NMR Refinement
MethodDetailsSoftware
distance geometry torsion angle dynamics simulated annealingNMRPipe
NMR Ensemble Information
Conformer Selection Criteriastructures with the least restraint violations
Conformers Calculated Total Number100
Conformers Submitted Total Number20
Representative Model1 (fewest violations)
Additional NMR Experimental Information
DetailsThis structure was determined using GFT/RD NMR techniques
Computation: NMR Software
#ClassificationVersionSoftware NameAuthor
1processingNMRPipe2.1Delaglio, F.
2data analysisXEASY1.3.1Bartels, C.
3structure solutionDYANA1.5Guntert, P.
4refinementCYANA2.1Guntert, P.