2AYJ

Solution structure of 50S ribosomal protein L40e from Sulfolobus solfataricus


SOLUTION NMR
NMR Experiment
ExperimentTypeSample ContentsSolventIonic StrengthpHPressureTemperature (K)Spectrometer
13D_15N-separated_NOESY1mM sso5336, U-15N,13C: 450 mM NaCl, 25 mM Na2PO4, 1mM Benzamidine, 1xinhibitor cooktail, 0.01% NAN3, 95% H2O, 5% D2O95% H2O/5% D2O450 mM NaCl, 25 mM Phosphate buffer6.5ambinet298
23D_13C-separated_NOESY1mM sso5336, U-15N,13C: 450 mM NaCl, 25 mM Na2PO4, 1mM Benzamidine, 1xinhibitor cooktail, 0.01% NAN3, 95% H2O, 5% D2O95% H2O/5% D2O450 mM NaCl, 25 mM Phosphate buffer6.5ambinet298
NMR Spectrometer Information
SpectrometerManufacturerModelField Strength
1BrukerAVANCE600
NMR Refinement
MethodDetailsSoftware
simulated annealing1081 NOE-derived distance restraints and 34 torsion angle restraintsNMRPipe
NMR Ensemble Information
Conformer Selection Criteriastructures with the lowest energy
Conformers Calculated Total Number100
Conformers Submitted Total Number20
Representative Model1 (lowest energy)
Computation: NMR Software
#ClassificationVersionSoftware NameAuthor
1processingNMRPipe2.3Delaglio, F.
2structure solutionCYANA2.1Gunter, P.
3refinementCNS1.0Brunger, A.T. et al