| NMR Experiment |
|---|
| Experiment | Type | Sample Contents | Solvent | Ionic Strength | pH | Pressure | Temperature (K) | Spectrometer |
|---|
| 1 | 3D_13C-separated_NOESY | 3.3 mM Class I aptamer U-15N, 13C; 3.63 mM GTP ligand NA; 10 % phosphate buffer NA, 95% H2O, 5% D2O | 95% H2O/5% D2O | 5 mM MgCl2, 75 mM KCl | 6.1 | ambient | 288 | |
| 2 | 3D_15N-separated_NOESY | 3.3 mM Class I aptamer U-15N, 13C; 3.63 mM GTP ligand NA; 10 % phosphate buffer NA, 95% H2O, 5% D2O | 95% H2O/5% D2O | 5 mM MgCl2, 75 mM KCl | 6.1 | ambient | 288 | |
| 3 | 2D NOESY | 3.3 mM Class I aptamer U-15N, 13C; 3.63 mM GTP ligand NA; 10 % phosphate buffer NA, 95% H2O, 5% D2O | 95% H2O/5% D2O | 5 mM MgCl2, 75 mM KCl | 6.1 | ambient | 288 | |
| 4 | DQF-COSY | 0.9 mM Class I aptamer NA; 0.99 nM GTP ligand U-15N, 13C; 10 % phospate buffer NA, 95% H2O, 5% D2O | 95% H2O/5% D2O | 5 mM MgCl2, 75 mM KCl | 6.1 | ambient | 288 | |
| 5 | 2D 1H decoupled 13C-1H CT HSQC | 0.8 mM Class I RNA aptamer U-15N,13C, 0.9 mM GTP NA, 10% phosphate buffer NA, 95% H2O, 5% D2O | 95% H2O/5% D2O | 5 mM MgCl2, 75 mM KCl | 6.8 | ambient | 303 | |
| 6 | 2D 13C-1H CT-TROSY | 0.8 mM Class I RNA aptamer U-15N,13C, 0.9 mM GTP NA, 10% phosphate buffer NA, 95% H2O, 5% D2O | 95% H2O/5% D2O | 5 mM MgCl2, 75 mM KCl | 6.8 | ambient | 303 | |
