2AJE
Solution structure of the Arabidopsis thaliana telomeric repeat-binding protein DNA binding domain
SOLUTION NMR
NMR Experiment | ||||||||
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Experiment | Type | Sample Contents | Solvent | Ionic Strength | pH | Pressure | Temperature (K) | Spectrometer |
1 | 3D_15N-separated_NOESY | 2mM protein in 50mM phosphate buffer, 150mM NaCl; 90% H2O, 10% D2O | 90% H2O/10% D2O | 50mM phosphate buffer | 7.4 | 1 atm | 298 | |
2 | 3D_13C-separated_NOESY | 2mM protein in 50mM phosphate buffer, 150mM NaCl; 90% H2O, 10% D2O | 90% H2O/10% D2O | 50mM phosphate buffer | 7.4 | 1 atm | 298 |
NMR Spectrometer Information | |||
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Spectrometer | Manufacturer | Model | Field Strength |
1 | Bruker | AVANCE | 500 |
2 | Bruker | AVANCE | 600 |
NMR Refinement | ||
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Method | Details | Software |
simulated annealing | ARIA |
NMR Ensemble Information | |
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Conformer Selection Criteria | structures with the lowest energy |
Conformers Calculated Total Number | 100 |
Conformers Submitted Total Number | 15 |
Representative Model | 1 (lowest energy) |
Computation: NMR Software | ||||
---|---|---|---|---|
# | Classification | Version | Software Name | Author |
1 | refinement | ARIA | 1.2 | Nilges, M. |
2 | structure solution | CYANA | 2.0 | Guntert, P. |
3 | collection | XwinNMR | 3.5 | Bruker |
4 | data analysis | AURELIA | 3.1.6 | Bruker |