1ZYI

Solution structure of ICLN, a multifunctional protein involved in regulatory mechanisms as different as cell volume regulation and rna splicing

SOLUTION NMR

NMR Experiment
Experiment	Type	Sample Contents	Solvent	Ionic Strength	pH	Pressure	Temperature (K)	Spectrometer
1	3D_13C-separated_NOESY	1mM ICln, 150mM NaCl, 25mM phosphate buffer.	90% H2O/10% D2O	25mM POTASSIUM PHOSPHATE, 150mM NaCl.	7.0	ambient	298
2	3D_15N-separated_NOESY	1mM ICln, 150mM NaCl, 25mM phosphate buffer.	90% H2O/10% D2O	25mM POTASSIUM PHOSPHATE, 150mM NaCl.	7.0	ambient	298
3	3D_CCH-TOCSY-NNH	1mM ICln, 150mM NaCl, 25mM phosphate buffer.	90% H2O/10% D2O	25mM POTASSIUM PHOSPHATE, 150mM NaCl.	7.0	ambient	298
4	3D_HCCH-TOCSY-NNH	1mM ICln, 150mM NaCl, 25mM phosphate buffer.	90% H2O/10% D2O	25mM POTASSIUM PHOSPHATE, 150mM NaCl.	7.0	ambient	298
5	3D_TOCSY-HSQC	1mM ICln, 150mM NaCl, 25mM phosphate buffer.	90% H2O/10% D2O	25mM POTASSIUM PHOSPHATE, 150mM NaCl.	7.0	ambient	298

NMR Spectrometer Information
Spectrometer	Manufacturer	Model	Field Strength
1	Varian	INOVA	800
2	Varian	INOVA	500

NMR Refinement
Method	Details	Software
simulated annealing		X-PLOR-NIH

NMR Ensemble Information
Conformer Selection Criteria	structures with the least restraint violations and structures with the lowest energy
Conformers Calculated Total Number	120
Conformers Submitted Total Number	15

Computation: NMR Software
#	Classification	Version	Software Name	Author
1	refinement	X-PLOR-NIH	2.11	Schwieters, C.D., Kuszewski, J.J., Tjandra, N., Clore, M.,

RCSB PDB Core Operations are funded by the U.S. National Science Foundation (DBI-2321666), the US Department of Energy (DE-SC0019749), and the National Cancer Institute, National Institute of Allergy and Infectious Diseases, and National Institute of General Medical Sciences of the National Institutes of Health under grant R01GM133198.