1ZWV

SOLUTION NMR

NMR Experiment
Experiment	Type	Sample Contents	Solvent	Ionic Strength	pH	Pressure	Temperature (K)	Spectrometer
1	2D NOESY	1.5mM w/wo U-15N, 13C; 50mM Phosphate buffer	90% H2O/10% D2O	100mM NaCl	7.5	ambient	295
2	3D_13C-separated_NOESY	1.5mM w/wo U-15N, 13C; 50mM Phosphate buffer	90% H2O/10% D2O	100mM NaCl	7.5	ambient	295
3	3D_15N-separated_NOESY	1.5mM w/wo U-15N, 13C; 50mM Phosphate buffer	90% H2O/10% D2O

NMR Spectrometer Information
Spectrometer	Manufacturer	Model	Field Strength
1	Bruker	AVANCE	500
2	Bruker	AVANCE	600

NMR Ensemble Information
Conformer Selection Criteria	target function
Conformers Calculated Total Number	100
Conformers Submitted Total Number	20
Representative Model	1 (lowest energy)