1Z30
NMR structure of the apical part of stemloop D from cloverleaf 1 of bovine enterovirus 1 RNA
SOLUTION NMR
| NMR Spectrometer Information | |||
|---|---|---|---|
| Spectrometer | Manufacturer | Model | Field Strength |
| 1 | Varian | INOVA | 600 |
| 2 | Varian | INOVA | 750 |
| NMR Refinement | ||
|---|---|---|
| Method | Details | Software |
| distance geometry, simulated annealing, energy minimisation | 518 upper limit distance constraints derived from NOE cross peaks, 184 torsion angle constraints defining 156 torsion angles, 36 hydrogen bond constraints | VNMR |
| NMR Ensemble Information | |
|---|---|
| Conformer Selection Criteria | structures with the lowest energy, target function |
| Conformers Calculated Total Number | 100 |
| Conformers Submitted Total Number | 15 |
| Representative Model | 5 (closest to the average) |
| Computation: NMR Software | ||||
|---|---|---|---|---|
| # | Classification | Version | Software Name | Author |
| 1 | collection | VNMR | 6.1, rev. c | Varian Inc. |
| 2 | processing | VNMR | 6.1, rev. c | Varian Inc. |
| 3 | data analysis | XEASY | 1.3.9 | Bartels |
| 4 | structure solution | CYANA | 1.0.6 | Herrmann |
| 5 | structure solution | FOUND | implemented in CYANA-1.0.6 | Guntert |
| 6 | refinement | OPAL | 2.6 | Luginbuhl |
