1Z2J

Solution structure of the HIV-1 frameshift inducing element


SOLUTION NMR
NMR Experiment
ExperimentTypeSample ContentsSolventIonic StrengthpHPressureTemperature (K)Spectrometer
12D NOESYAll NMR samples were 1 mM RNA, and approximately 50 mM NaCl100% D2O50 mM NaCl6.81 atm303
22D TOCSYAll NMR samples were 1 mM RNA, and approximately 50 mM NaCl100% D2O50 mM NaCl6.81 atm303
33D_13C-separated_NOESYAll NMR samples were 1 mM RNA, and approximately 50 mM NaCl100% D2O50 mM NaCl6.81 atm303
42D NOESYAll NMR samples were 1 mM RNA, and approximately 50 mM NaCl90% H2O/10% D2O50 mM NaCl6.81 atm283
5HNN-COSYAll NMR samples were 1 mM RNA, and approximately 50 mM NaCl90% H2O/10% D2O50 mM NaCl6.81 atm283
6HCCH-TOCSYAll NMR samples were 1 mM RNA, and approximately 50 mM NaCl100% D2O50 mM NaCl6.81 atm303
NMR Spectrometer Information
SpectrometerManufacturerModelField Strength
1BrukerDMX600
2BrukerDMX750
3VarianINOVA600
4VarianINOVA800
5VarianINOVA900
NMR Ensemble Information
Conformer Selection Criteriastructures with the least restraint violations,structures with the lowest energy
Conformers Calculated Total Number100
Conformers Submitted Total Number20
Representative Model1 (lowest energy)
Computation: NMR Software
#ClassificationVersionSoftware NameAuthor
1collectionXwinNMR
2processingNMRPipeDelaglio
3data analysisSparky
4structure solutionCNSBrunger
5refinementXPLOR-NIHSchwieters