1YUB

SOLUTION STRUCTURE OF AN RRNA METHYLTRANSFERASE (ERMAM) THAT CONFERS MACROLIDE-LINCOSAMIDE-STREPTOGRAMIN ANTIBIOTIC RESISTANCE, NMR, MINIMIZED AVERAGE STRUCTURE

SOLUTION NMR

NMR Experiment
Experiment	Type	Sample Contents	Solvent	Ionic Strength	pH	Pressure	Temperature (K)	Spectrometer
1	2D			50 mM SODIUM PHOSPHATE, 100 mM NACL	6.5		303
2	3D			50 mM SODIUM PHOSPHATE, 100 mM NACL	6.5		303
3	4D NMR			50 mM SODIUM PHOSPHATE, 100 mM NACL	6.5		303

NMR Spectrometer Information
Spectrometer	Manufacturer	Model	Field Strength
1	Bruker	DMX500	500
2	Bruker	AMX600	600
3	Bruker	DRX800	800

NMR Refinement
Method	Details	Software
DG/SA	THE TOTAL NUMBER OF DISTANCE RESTRAINTS USED WAS 3259. THE TOTAL NUMBER OF TORSIONAL RESTRAINTS USED WAS 63.	X-PLOR

NMR Ensemble Information
Conformer Selection Criteria	LOWEST ENERGY AND LEAST RESTRAINT VIOLATION
Conformers Calculated Total Number	17
Conformers Submitted Total Number	1

Computation: NMR Software
#	Classification	Version	Software Name	Author
1	refinement	X-PLOR	3.1	BRUNGER
2	structure solution	X-PLOR	3.1

RCSB PDB Core Operations are funded by the National Science Foundation (DBI-1832184), the US Department of Energy (DE-SC0019749), and the National Cancer Institute, National Institute of Allergy and Infectious Diseases, and National Institute of General Medical Sciences of the National Institutes of Health under grant R01GM133198.