Experiment: 1YSV

SOLUTION NMR

NMR Experiment
Experiment	Type	Sample Contents	Solvent	Ionic Strength	pH	Pressure	Temperature (K)	Spectrometer
1	2D NOESY	1mM GluR-B R/G pre-mRNA; 10mM phosphate buffer, pH 6; 100% D2O	100% D2O	10mM sodium phosphate	6	ambient	303
2	3D_13C-separated_NOESY	1mM GluR-B R/G pre-mRNA U-15N,13C[A,C]; 10mM phosphate buffer, pH 6; 90% H2O, 10% D2O	90% H2O/10% D2O	10mM sodium phosphate	6	ambient	303
3	3D_13C-separated_NOESY	1mM GluR-B R/G pre-mRNA U-15N,13C[G,U]; 10mM phosphate buffer, pH 6; 100% D2O	100% D2O	10mM sodium phosphate	6	ambient	303
4	t2-coupled HSQC	1mM GluR-B R/G pre-mRNA U-15N,13C[A,C]; 10mM phosphate buffer, pH 6; 90% H2O, 10% D2O	90% H2O/10% D2O	10mM sodium phosphate	6	ambient	303
5	t2-coupled HSQC	1mM GluR-B R/G pre-mRNA U-15N,13C[G,U]; 10mM phosphate buffer, pH 6; 100% D2O	100% D2O	10mM sodium phosphate	6	ambient	303

NMR Spectrometer Information
Spectrometer	Manufacturer	Model	Field Strength
1	Bruker	AVANCE	900
2	Bruker	DRX	750
3	Bruker	DRX	600
4	Bruker	DRX	500

NMR Ensemble Information
Conformer Selection Criteria	structures with the lowest energy
Conformers Calculated Total Number	30
Conformers Submitted Total Number	13
Representative Model	1 (lowest energy)

Computation: NMR Software
#	Classification	Version	Software Name	Author
1	refinement	Amber	7.0	Case, DA, et al.
2	processing	XwinNMR	3.5	Bruker

RCSB PDB Core Operations are funded by the U.S. National Science Foundation (DBI-2321666), the US Department of Energy (DE-SC0019749), and the National Cancer Institute, National Institute of Allergy and Infectious Diseases, and National Institute of General Medical Sciences of the National Institutes of Health under grant R01GM133198.