1Y4O
Solution structure of a mouse cytoplasmic Roadblock/LC7 dynein light chain
SOLUTION NMR
NMR Experiment | ||||||||
---|---|---|---|---|---|---|---|---|
Experiment | Type | Sample Contents | Solvent | Ionic Strength | pH | Pressure | Temperature (K) | Spectrometer |
1 | 3D HNCACB | 10mM Sodium Acetate, 100mM NaCl, 0.02% NaN3,90% H2O, 10% D2O | 90% H2O/10% D2O | 5.0 | 1 atm | 298.2 | ||
2 | 3D CBCA(CO)NH | 10mM Sodium Acetate, 100mM NaCl, 0.02% NaN3,90% H2O, 10% D2O | 90% H2O/10% D2O | 5.0 | 1 atm | 298.2 | ||
3 | 3D HCCH-TOCSY | 10mM Sodium Acetate, 100mM NaCl, 0.02% NaN3,90% H2O, 10% D2O | 90% H2O/10% D2O | 5.0 | 1 atm | 298.2 | ||
4 | 3D HNCO | 10mM Sodium Acetate, 100mM NaCl, 0.02% NaN3,90% H2O, 10% D2O | 90% H2O/10% D2O | 5.0 | 1 atm | 298.2 | ||
5 | 3D_13C-separated_NOESY | 10mM Sodium Acetate, 100mM NaCl, 0.02% NaN3,90% H2O, 10% D2O | 90% H2O/10% D2O | 5.0 | 1 atm | 298.2 | ||
6 | 3D_15N-separated_NOESY | 10mM Sodium Acetate, 100mM NaCl, 0.02% NaN3,90% H2O, 10% D2O | 90% H2O/10% D2O | 5.0 | 1 atm | 298.2 |
NMR Spectrometer Information | |||
---|---|---|---|
Spectrometer | Manufacturer | Model | Field Strength |
1 | Varian | INOVA | 600 |
NMR Refinement | ||
---|---|---|
Method | Details | Software |
distance geometry simulated annealing torsion angle dynamics | VNMR |
NMR Ensemble Information | |
---|---|
Conformer Selection Criteria | target function |
Conformers Calculated Total Number | 100 |
Conformers Submitted Total Number | 20 |
Representative Model | 7 (lowest energy) |
Computation: NMR Software | ||||
---|---|---|---|---|
# | Classification | Version | Software Name | Author |
1 | collection | VNMR | 6.1 | Varian, Inc. |
2 | processing | NMRPipe | 97.027.12.56 | Delaglio et al. |
3 | data analysis | Sparky | 3.72 | Goddard T.D. and Kneller, D.G. |
4 | structure solution | CYANA | 1.0.6 | Guntert et al |
5 | refinement | XPLOR-NIH | 2.9.3 | Schwieters, C.D. et al |