1XZY

Solution structure of the P30-trans form of Alpha Hemoglobin Stabilizing Protein (AHSP)

SOLUTION NMR

NMR Experiment
Experiment	Type	Sample Contents	Solvent	Ionic Strength	pH	Pressure	Temperature (K)	Spectrometer
1	3D_15N-separated_NOESY	20mM sodium phosphate, 100mM NaCl, 90% H2O, 10% D2O	90% H2O/10% D2O	20mM phosphate, 100mM NaCl	6.9	ambient	288
2	2D NOESY	20mM sodium phosphate, 100mM NaCl, 90% H2O, 10% D2O	90% H2O/10% D2O	20mM phosphate, 100mM NaCl	6.9	ambient	288
3	3D_13C-separated_NOESY	20mM sodium phosphate, 100mM NaCl, 90% H2O, 10% D2O	90% H2O/10% D2O	20mM phosphate, 100mM NaCl	6.9	ambient	288

NMR Spectrometer Information
Spectrometer	Manufacturer	Model	Field Strength
1	Bruker	DRX	600
2	Bruker	DRX	800

NMR Refinement
Method	Details	Software
simulated annealing		CNS

NMR Ensemble Information
Conformer Selection Criteria	structures with the lowest energy
Conformers Calculated Total Number	100
Conformers Submitted Total Number	20

Additional NMR Experimental Information
Details	The structure was determined using triple-resonance NMR spectroscopy.

Computation: NMR Software
#	Classification	Version	Software Name	Author
1	structure solution	CNS	1	Brunger
2	structure solution	DYANA	1	Guntert
3	data analysis	Sparky	3.111	Goddard
4	refinement	DYANA	1	Guntert

RCSB PDB Core Operations are funded by the National Science Foundation (DBI-1832184), the US Department of Energy (DE-SC0019749), and the National Cancer Institute, National Institute of Allergy and Infectious Diseases, and National Institute of General Medical Sciences of the National Institutes of Health under grant R01GM133198.