1XDX

Solution Structure of the Tctex1 Light Chain From Chlamydomonas Inner Dynein Arm I1

SOLUTION NMR

NMR Experiment
Experiment	Type	Sample Contents	Solvent	Ionic Strength	pH	Pressure	Temperature (K)	Spectrometer
1	3D_15N-separated_NOESY	~0.7 mM tctex1 protein; U-15N,13C; 20 mM Na phosphate; pH 6.7; 100mM NaCl; 20mM DTT; 90% H2O, 10% D2O	90% H2O/10% D2O		6.7	ambient	298
2	3D_13C-separated_NOESY	~0.7 mM tctex1 protein; U-15N,13C; 20 mM Na phosphate; pH 6.7; 100mM NaCl; 20mM DTT; >99% D2O	>99% D2O

NMR Spectrometer Information
Spectrometer	Manufacturer	Model	Field Strength
1	Varian	INOVA	500
2	Varian	INOVA	600

NMR Refinement
Method	Details	Software
torsion angle dynamics simulated annealing		VNMR

NMR Ensemble Information
Conformer Selection Criteria	target function,structures with the least restraint violations
Conformers Calculated Total Number	90
Conformers Submitted Total Number	15
Representative Model	1 (fewest violations)

Additional NMR Experimental Information
Details	The structure was determined using triple-resonance NMR spectroscopy.

Computation: NMR Software
#	Classification	Version	Software Name	Author
1	collection	VNMR	6.1B	varian
2	processing	NMRPipe	1.1	Delaglio, F.
3	data analysis	XEASY	1.4	Bartels, C.
4	structure solution	CYANA	1.1	Guentert, P.
5	refinement	X-PLOR	3.851	Brunger

RCSB PDB Core Operations are funded by the U.S. National Science Foundation (DBI-2321666), the US Department of Energy (DE-SC0019749), and the National Cancer Institute, National Institute of Allergy and Infectious Diseases, and National Institute of General Medical Sciences of the National Institutes of Health under grant R01GM133198.