1X65
Solution structure of the third cold-shock domain of the human KIAA0885 protein (UNR PROTEIN)
SOLUTION NMR
| NMR Experiment | ||||||||
|---|---|---|---|---|---|---|---|---|
| Experiment | Type | Sample Contents | Solvent | Ionic Strength | pH | Pressure | Temperature (K) | Spectrometer |
| 1 | 3D_15N-separated_NOESY | 0.67mM COLD-SHOCK DOMAIN, 20mM Phosphate buffer Na, 100mM NaCl, 1mM d-DTT, 0.02% NaN3 | 90% H2O/10% D2O | 120 mM | 6.0 | ambient | 298 | |
| 2 | 3D_13C-separated_NOESY | 0.67mM COLD-SHOCK DOMAIN, 20mM Phosphate buffer Na, 100mM NaCl, 1mM d-DTT, 0.02% NaN3 | 90% H2O/10% D2O | 120 mM | 6.0 | ambient | 298 | |
| NMR Spectrometer Information | |||
|---|---|---|---|
| Spectrometer | Manufacturer | Model | Field Strength |
| 1 | Bruker | Avance | 600 |
| NMR Refinement | ||
|---|---|---|
| Method | Details | Software |
| torsion angle dynamics | XWINNMR | |
| NMR Ensemble Information | |
|---|---|
| Conformer Selection Criteria | structures with the least restraint violations |
| Conformers Calculated Total Number | 100 |
| Conformers Submitted Total Number | 20 |
| Representative Model | 1 (fewest violations) |
| Computation: NMR Software | ||||
|---|---|---|---|---|
| # | Classification | Version | Software Name | Author |
| 1 | collection | XWINNMR | 3.5 | BRUKER |
| 2 | processing | NMRPipe | 20020425 | FRANK DELAGLIO |
| 3 | data analysis | NMRVIEW | 5.0.4 | BRUCE A. JOHNSON |
| 4 | data analysis | KUJIRA | 0.899a | NAOHIRO KOBAYASHI |
| 5 | refinement | CYANA | 2.0.26 | PETER GUNTERT |
