1WXL
Solution Structure of the HMG-box domain in the SSRP1 subunit of FACT
SOLUTION NMR
| NMR Experiment | ||||||||
|---|---|---|---|---|---|---|---|---|
| Experiment | Type | Sample Contents | Solvent | Ionic Strength | pH | Pressure | Temperature (K) | Spectrometer |
| 1 | 3D_15N-separated_NOESY | 2.2mM Protein U-15N,13C; 2mM Sodium Acetate buffer; 90% H2O,10% D2O | 90% H2O/10% D2O | 0 | 5.2 | ambient | 298 | |
| 2 | 3D_13C-separated_NOESY | 2.2mM Protein U-15N,13C; 2mM Sodium Acetate buffer; 100% D2O | 100% D2O | 0 | 5.2 | ambient | 298 | |
| 3 | 2D TOCSY | 2.2mM Protein U-15N,13C; 2mM Sodium Acetate buffer; 100% D2O | 100% D2O | 0 | 5.2 | ambient | 298 | |
| NMR Spectrometer Information | |||
|---|---|---|---|
| Spectrometer | Manufacturer | Model | Field Strength |
| 1 | Bruker | DMX | 600 |
| 2 | Bruker | DMX | 750 |
| NMR Refinement | ||
|---|---|---|
| Method | Details | Software |
| simulated annealing | XwinNMR | |
| NMR Ensemble Information | |
|---|---|
| Conformer Selection Criteria | structures with the lowest energy |
| Conformers Calculated Total Number | 100 |
| Conformers Submitted Total Number | 30 |
| Representative Model | 1 (lowest energy) |
| Computation: NMR Software | ||||
|---|---|---|---|---|
| # | Classification | Version | Software Name | Author |
| 1 | collection | XwinNMR | 2.6 | bruker |
| 2 | processing | NMRPipe | Delaglio et al. | |
| 3 | structure solution | CNS | 1.1 | Brunger et al. |
| 4 | data analysis | PIPP | Garrett et al. | |
| 5 | refinement | CNS | 1.1 | Brunger et al. |
