1WVK

NMR Solution Structure of the Partially Disordered Protein At2g23090 from Arabidopsis thaliana

SOLUTION NMR

NMR Experiment
Experiment	Type	Sample Contents	Solvent	Ionic Strength	pH	Pressure	Temperature (K)	Spectrometer
1	3D_13C-separated_NOESY	1mM At2g23090 U-13C, U-15N; 10mM Bis-Tris buffer, 100mM NaCl, 90% H2O, 10% D2O	10% D2O, 90% H2O	100mM NaCl	5.8	ambient	300
2	3D_15N-separated_NOESY	1mM At2g23090 U-13C, U-15N; 10mM Bis-Tris buffer, 100mM NaCl, 90% H2O, 10% D2O	10% D2O, 90% H2O	100mM NaCl	5.8	ambient	300

NMR Spectrometer Information
Spectrometer	Manufacturer	Model	Field Strength
1	Varian	INOVA	900
2	Varian	INOVA	600

NMR Refinement
Method	Details	Software
simulated annealing, molecular dynamics, matrix relaxation, torsion angle dynamics, water refinement		ARIA

NMR Ensemble Information
Conformer Selection Criteria	structures with the lowest energy
Conformers Calculated Total Number	15
Conformers Submitted Total Number	15
Representative Model	1 (lowest energy)

Computation: NMR Software
#	Classification	Version	Software Name	Author
1	structure solution	ARIA	1.2	Nilges, M.
2	refinement	ARIA	1.2	Nilges, M.

RCSB PDB Core Operations are funded by the U.S. National Science Foundation (DBI-2321666), the US Department of Energy (DE-SC0019749), and the National Cancer Institute, National Institute of Allergy and Infectious Diseases, and National Institute of General Medical Sciences of the National Institutes of Health under grant R01GM133198.