1WIJ
Solution Structure of the DNA-Binding Domain of Ethylene-Insensitive3-Like3
SOLUTION NMR
| NMR Experiment | ||||||||
|---|---|---|---|---|---|---|---|---|
| Experiment | Type | Sample Contents | Solvent | Ionic Strength | pH | Pressure | Temperature (K) | Spectrometer |
| 1 | 2D NOESY | 1mM EIL3-DBD; 20mM KPi; 200mM KCl; 95% H2O; 5% D2O | 200mM | 6.0 | 1 atm | 298 | ||
| 2 | 2D TOCSY | 1mM EIL3-DBD; 20mM KPi; 200mM KCl; 95% H2O; 5% D2O | 200mM | 6.0 | 1 atm | 298 | ||
| 3 | 3D_13C-separated_NOESY | 1mM EIL3-DBD; 20mM KPi; 200mM KCl; 95% H2O; 5% D2O | 200mM | 6.0 | 1 atm | 298 | ||
| 4 | 3D_15N-separated_NOESY | 1mM EIL3-DBD; 20mM KPi; 200mM KCl; 95% H2O; 5% D2O | 200mM | 6.0 | 1 atm | 298 | ||
| 5 | DQF-COSY | 1mM EIL3-DBD; 20mM KPi; 200mM KCl; 95% H2O; 5% D2O | 200mM | 6.0 | 1 atm | 298 | ||
| 6 | HMQC-J | 1mM EIL3-DBD; 20mM KPi; 200mM KCl; 95% H2O; 5% D2O | 200mM | 6.0 | 1 atm | 298 | ||
| NMR Spectrometer Information | |||
|---|---|---|---|
| Spectrometer | Manufacturer | Model | Field Strength |
| 1 | Bruker | DMX | 750 |
| 2 | Bruker | DMX | 500 |
| NMR Refinement | ||
|---|---|---|
| Method | Details | Software |
| simulated annealing | Felix | |
| NMR Ensemble Information | |
|---|---|
| Conformer Selection Criteria | structures with the lowest energy |
| Conformers Calculated Total Number | 50 |
| Conformers Submitted Total Number | 20 |
| Representative Model | 17 (lowest energy) |
| Computation: NMR Software | ||||
|---|---|---|---|---|
| # | Classification | Version | Software Name | Author |
| 1 | data analysis | Felix | 2000 | |
| 2 | structure solution | CNS | 1.1 | |
| 3 | refinement | CNS | 1.1 | |
