1WI8

Solution structure of the RNA binding domain of eukaryotic initiation factor 4B

SOLUTION NMR

NMR Experiment
Experiment	Type	Sample Contents	Solvent	Ionic Strength	pH	Pressure	Temperature (K)	Spectrometer
1	3D_13C-separated_NOESY	1.0mM 13C/15N-PROTEIN 20mM d-Tris-HCl; 100mM NaCl; 1mM d-DTT; 0.02% NaN3	90% H2O/10% D2O	100mM	7.0	ambient	298
2	3D_15N-separated_NOESY	1.0mM 13C/15N-PROTEIN 20mM d-Tris-HCl; 100mM NaCl; 1mM d-DTT; 0.02% NaN3	90% H2O/10% D2O	100mM	7.0	ambient	298

NMR Spectrometer Information
Spectrometer	Manufacturer	Model	Field Strength
1	Bruker	AVANCE	800

NMR Refinement
Method	Details	Software
torsion angle dynamics, simulated annealing		XwinNMR

NMR Ensemble Information
Conformer Selection Criteria	target function
Conformers Calculated Total Number	100
Conformers Submitted Total Number	20
Representative Model	1 (lowest energy)

Additional NMR Experimental Information
Details	The structure was determined using triple-resonance NMR spectroscopy.

Computation: NMR Software
#	Classification	Version	Software Name	Author
1	collection	XwinNMR	3.5	Bruker
2	processing	NMRPipe	2.3	Delaglio, F.
3	data analysis	NMRView	5.0.4	Johnson, B.A.
4	data analysis	KUJIRA	0.901	Kobayashi, N.
5	data analysis	Olivia	1.9.12	Yokochi, M.
6	structure solution	CYANA	2.0.17	Guentert, P.
7	refinement	CYANA	2.0.17	Guentert, P.

RCSB PDB Core Operations are funded by the U.S. National Science Foundation (DBI-2321666), the US Department of Energy (DE-SC0019749), and the National Cancer Institute, National Institute of Allergy and Infectious Diseases, and National Institute of General Medical Sciences of the National Institutes of Health under grant R01GM133198.