1WHY

Solution structure of the RNA recognition motif from hypothetical RNA binding protein BC052180


SOLUTION NMR
NMR Experiment
ExperimentTypeSample ContentsSolventIonic StrengthpHPressureTemperature (K)Spectrometer
13D_13C-separated_NOESY1.13mM PROTEIN U-15N, 13C; 20mM phosphate buffer NA; 100mM NaCl; 1mM DTT; 0.02% NaN3; 90% H2O; 10% D2O90% H2O/10% D2O100mM6.0ambient298
23D_15N-separated_NOESY1.13mM PROTEIN U-15N, 13C; 20mM phosphate buffer NA; 100mM NaCl; 1mM DTT; 0.02% NaN3; 90% H2O; 10% D2O90% H2O/10% D2O100mM6.0ambient298
NMR Spectrometer Information
SpectrometerManufacturerModelField Strength
1BrukerAVANCE800
NMR Refinement
MethodDetailsSoftware
torsion angle dynamics, simulated annealingNMRView
NMR Ensemble Information
Conformer Selection Criteriatarget function
Conformers Calculated Total Number100
Conformers Submitted Total Number20
Representative Model1 (lowest energy)
Additional NMR Experimental Information
DetailsThe structure was determined using triple-resonance NMR spectroscopy.
Computation: NMR Software
#ClassificationVersionSoftware NameAuthor
1data analysisNMRView5.0.4Johnson
2structure solutionCYANA2.0Guentert
3processingNMRPipe2.3Delaglio
4data analysisKUJIRA0.901Kobayashi, N.
5refinementCYANA2.0Guentert