1WH5

Solution structure of homeobox domain of Arabidopsisthaliana zinc finger homeobox family protein


SOLUTION NMR
NMR Experiment
ExperimentTypeSample ContentsSolventIonic StrengthpHPressureTemperature (K)Spectrometer
13D_13C-separated_NOESY1.1mM homeobox domain U-15N, 13C; 20mM phosphate buffer NA; 100mM NaCl; 1mM d-DTT; 0.02% NaN3; 10% D2O90% H2O/10% D2O120mM6.0ambient298
23D_15N-separated_NOESY1.1mM homeobox domain U-15N, 13C; 20mM phosphate buffer NA; 100mM NaCl; 1mM d-DTT; 0.02% NaN3; 10% D2O90% H2O/10% D2O120mM6.0ambient298
NMR Spectrometer Information
SpectrometerManufacturerModelField Strength
1BrukerAVANCE800
NMR Refinement
MethodDetailsSoftware
torsion angle dynamicsXwinNMR
NMR Ensemble Information
Conformer Selection Criteriastructures with the least restraint violations, target function
Conformers Calculated Total Number100
Conformers Submitted Total Number20
Representative Model1 (lowest energy)
Computation: NMR Software
#ClassificationVersionSoftware NameAuthor
1collectionXwinNMR2.6Bruker
2processingNMRPipe20020425Delaglio, F.
3data analysisNMRView5.0.4Johnson, B.A.
4data analysisKUJIRA0.811Kobayashi, N.
5structure solutionCYANA1.0.7Guentert, P.
6refinementCYANA1.0.7Guentert, P.