SOLUTION NMR
NMR Experiment
ExperimentTypeSample ContentsSolventIonic StrengthpHPressureTemperature (K)Spectrometer
13D_13C-separated_NOESY1.17mM 13C/15N-labeled protein; 20mM PiNa, 100mM NaCl, 1mM DTT, 0.02% NaN390% H2O/10% D2O120mM6.0ambient298
23D_15N-separated_NOESY1.17mM 13C/15N-labeled protein; 20mM PiNa, 100mM NaCl, 1mM DTT, 0.02% NaN390% H2O/10% D2O120mM6.0ambient298
NMR Spectrometer Information
SpectrometerManufacturerModelField Strength
1VarianINOVA800
NMR Refinement
MethodDetailsSoftware
torsion angle dynamicsVNMR
NMR Ensemble Information
Conformer Selection Criteriastructures with the least restraint violations, target function
Conformers Calculated Total Number100
Conformers Submitted Total Number20
Representative Model1 (lowest energy)
Computation: NMR Software
#ClassificationVersionSoftware NameAuthor
1collectionVNMR6.1CVarian
2processingNMRPipe20020425Delaglio, F.
3data analysisNMRView5.0.4Johnson, B.A.
4data analysisKUJIRA0.8992Kobayashi, N.
5structure solutionCYANA1.0.7Guentert, P.
6refinementCYANA1.0.7Guentert, P.