1WEP

Solution structure of PHD domain in PHF8


SOLUTION NMR
NMR Experiment
ExperimentTypeSample ContentsSolventIonic StrengthpHPressureTemperature (K)Spectrometer
13D_15N-separated_NOESY0.8mM U-15, 13C; 20mM d-Tris-HCl(pH 7.0); 100mM NaCl; 0.1mM ZnCl2; 1mM d-DTT; 0.02% NaN3; 90% H2O, 10% D2O90% H2O/10% D2O120mM7.0ambient293
23D_13C-separated_NOESY0.8mM U-15, 13C; 20mM d-Tris-HCl(pH 7.0); 100mM NaCl; 0.1mM ZnCl2; 1mM d-DTT; 0.02% NaN3; 90% H2O, 10% D2O90% H2O/10% D2O120mM7.0ambient293
NMR Spectrometer Information
SpectrometerManufacturerModelField Strength
1BrukerAVANCE800
NMR Refinement
MethodDetailsSoftware
torsion angle dynamicsXwinNMR
NMR Ensemble Information
Conformer Selection Criteriastructures with the least restraint violations, structures with the lowest energy, target function
Conformers Calculated Total Number100
Conformers Submitted Total Number20
Representative Model1 (lowest energy)
Computation: NMR Software
#ClassificationVersionSoftware NameAuthor
1collectionXwinNMR2.6Bruker
2processingNMRPipe20020425Delaglio,F.
3data analysisNMRView5.0.4Johnson,B.A.
4data analysisKUJIRA0.863Cobayashi,N.
5structure solutionCYANA2.0.17Guentert,P.
6refinementCYANA2.0.17Guentert,P.