1VA3
Solution Structure of Transcription Factor Sp1 DNA Binding Domain (Zinc Finger 3)
SOLUTION NMR
| NMR Experiment | ||||||||
|---|---|---|---|---|---|---|---|---|
| Experiment | Type | Sample Contents | Solvent | Ionic Strength | pH | Pressure | Temperature (K) | Spectrometer |
| 1 | 3D_13C-separated_NOESY | 1mM Sp1 DNA-Binding Domain U-15N/13C; 10mM Tris-d; 50mM NaCl; 1mM DTT | 90% H2O/10% D2O | 10mM Tris-d, 50mM NaCl | 7.4 | 1 atm | 298 | |
| 2 | 3D_15N-separated_NOESY | 1mM Sp1 DNA-Binding Domain U-15N/13C; 10mM Tris-d; 50mM NaCl; 1mM DTT | 90% H2O/10% D2O | 10mM Tris-d, 50mM NaCl | 7.4 | 1 atm | 298 | |
| NMR Spectrometer Information | |||
|---|---|---|---|
| Spectrometer | Manufacturer | Model | Field Strength |
| 1 | Varian | INOVA | 600 |
| NMR Refinement | ||
|---|---|---|
| Method | Details | Software |
| torsion angle dynamics, simulated annealing | CNS | |
| NMR Ensemble Information | |
|---|---|
| Conformer Selection Criteria | structures with the lowest energy |
| Conformers Calculated Total Number | 100 |
| Conformers Submitted Total Number | 31 |
| Representative Model | 31 (minimized average structure) |
| Additional NMR Experimental Information | |
|---|---|
| Details | The structure was determined using triple-resonance NMR spectroscopy. |
| Computation: NMR Software | ||||
|---|---|---|---|---|
| # | Classification | Version | Software Name | Author |
| 1 | structure solution | CNS | 1.1 | |
| 2 | processing | NMRPipe | 2.1 | F. Delaglio, et al. |
| 3 | data analysis | NMRView | 5.0.4 | Bruce A. Johnson |
| 4 | refinement | CNS | 1.1 | |
