1VA2

Solution Structure of Transcription Factor Sp1 DNA Binding Domain (Zinc Finger 2)


SOLUTION NMR
NMR Experiment
ExperimentTypeSample ContentsSolventIonic StrengthpHPressureTemperature (K)Spectrometer
13D_13C-separated_NOESY1mM Sp1 DNA-Binding Domain U-15N/13C; 10mM Tris-d; 50mM NaCl; 1mM DTT90% H2O/10% D2O10mM Tris-d, 50mM NaCl7.41 atm298
23D_15N-separated_NOESY1mM Sp1 DNA-Binding Domain U-15N/13C; 10mM Tris-d; 50mM NaCl; 1mM DTT90% H2O/10% D2O10mM Tris-d, 50mM NaCl7.41 atm298
NMR Spectrometer Information
SpectrometerManufacturerModelField Strength
1VarianINOVA600
NMR Refinement
MethodDetailsSoftware
torsion angle dynamics, simulated annealingCNS
NMR Ensemble Information
Conformer Selection Criteriastructures with the lowest energy
Conformers Calculated Total Number100
Conformers Submitted Total Number31
Representative Model31 (minimized average structure)
Additional NMR Experimental Information
DetailsThe structure was determined using triple-resonance NMR spectroscopy.
Computation: NMR Software
#ClassificationVersionSoftware NameAuthor
1structure solutionCNS1.1
2processingNMRPipe2.1F. Delaglio, et al.
3data analysisNMRView5.0.4Bruce A. Johnson
4refinementCNS1.1