1UEL
Solution structure of ubiquitin-like domain of hHR23B complexed with ubiquitin-interacting motif of proteasome subunit S5a
SOLUTION NMR
| NMR Spectrometer Information | |||
|---|---|---|---|
| Spectrometer | Manufacturer | Model | Field Strength |
| 1 | Bruker | DRX | 500 |
| 2 | Bruker | DRX | 800 |
| NMR Refinement | ||
|---|---|---|
| Method | Details | Software |
| simulated annealing procedure in CNS and further refined by AMBER 7 | RESIDUES INCLUDING MET1 THROUGH THR75 OF HHR23B AND LEU278 THROUGH GLN 296 OF S5A ARE ORDERED IN SOLUTION, WHILE THE REST IS HIGHLY FLEXIBLE (BASED ON 1H-15N HETERONUCLEAR NOE ANALYSIS). | Amber |
| NMR Ensemble Information | |
|---|---|
| Conformer Selection Criteria | The submitted conformer models are the 20 structures with the lowest |
| Conformers Calculated Total Number | 100 |
| Conformers Submitted Total Number | 20 |
| Computation: NMR Software | ||||
|---|---|---|---|---|
| # | Classification | Version | Software Name | Author |
| 1 | refinement | Amber | 7 | |
