1TI3

Solution structure of the Thioredoxin h1 from poplar, a CPPC active site variant


SOLUTION NMR
NMR Experiment
ExperimentTypeSample ContentsSolventIonic StrengthpHPressureTemperature (K)Spectrometer
13D_13C-separated_NOESY1.85 mM Trxh1, 50 mM phosphate buffer, 95% H2O, 5% D2O95% H2O/5% D2O5.9298
23D_15N-separated_NOESY1.85 mM Trxh1, 50 mM phosphate buffer, 95% H2O, 5% D2O95% H2O/5% D2O5.9298
NMR Spectrometer Information
SpectrometerManufacturerModelField Strength
1BrukerDRX600
NMR Ensemble Information
Conformer Selection Criteriastructures with the lowest energy
Conformers Calculated Total Number50
Conformers Submitted Total Number20
Representative Model1 (lowest energy)
Computation: NMR Software
#ClassificationVersionSoftware NameAuthor
1structure solutionDIANA
2refinementDiscover