1TI3
Solution structure of the Thioredoxin h1 from poplar, a CPPC active site variant
SOLUTION NMR
NMR Experiment | ||||||||
---|---|---|---|---|---|---|---|---|
Experiment | Type | Sample Contents | Solvent | Ionic Strength | pH | Pressure | Temperature (K) | Spectrometer |
1 | 3D_13C-separated_NOESY | 1.85 mM Trxh1, 50 mM phosphate buffer, 95% H2O, 5% D2O | 95% H2O/5% D2O | 5.9 | 298 | |||
2 | 3D_15N-separated_NOESY | 1.85 mM Trxh1, 50 mM phosphate buffer, 95% H2O, 5% D2O | 95% H2O/5% D2O | 5.9 | 298 |
NMR Spectrometer Information | |||
---|---|---|---|
Spectrometer | Manufacturer | Model | Field Strength |
1 | Bruker | DRX | 600 |
NMR Ensemble Information | |
---|---|
Conformer Selection Criteria | structures with the lowest energy |
Conformers Calculated Total Number | 50 |
Conformers Submitted Total Number | 20 |
Representative Model | 1 (lowest energy) |
Computation: NMR Software | ||||
---|---|---|---|---|
# | Classification | Version | Software Name | Author |
1 | structure solution | DIANA | ||
2 | refinement | Discover |