1T2Y

NMR solution structure of the protein part of Cu6-Neurospora crassa MT


SOLUTION NMR
NMR Experiment
ExperimentTypeSample ContentsSolventIonic StrengthpHPressureTemperature (K)Spectrometer
12D TOCSY0.5 mM Neurospora crassa MT, 20 mM phosphate buffer, 0.1 mM 2,2-dimethyl-2-silapentane-5-sulfonate, 0.02% NaN3, 90% H2O, 10% D2O90% H2O/10% D2O20 mM KPi6.5ambient283
22D NOESY0.5 mM Neurospora crassa MT, 20 mM phosphate buffer, 0.1 mM 2,2-dimethyl-2-silapentane-5-sulfonate, 0.02% NaN3, 90% H2O, 10% D2O90% H2O/10% D2O20 mM KPi6.5ambient283
3DQF-COSY0.5 mM Neurospora crassa MT, 20 mM phosphate buffer, 0.1 mM 2,2-dimethyl-2-silapentane-5-sulfonate, 0.02% NaN3, 90% H2O, 10% D2O90% H2O/10% D2O20 mM KPi6.5ambient283
NMR Spectrometer Information
SpectrometerManufacturerModelField Strength
1VarianINOVA800
NMR Refinement
MethodDetailsSoftware
the hybrid distance geometry-dynamical simulated annealing protocolthe structure was calculated with 152 NOEs and 13 dihedral angle restraintsNMRPipe
NMR Ensemble Information
Conformer Selection Criteria
Conformers Calculated Total Number
Conformers Submitted Total Number1
Representative Model1 (closest to the average)
Additional NMR Experimental Information
DetailsThis structure was determined using standard 2D homonuclear techniques
Computation: NMR Software
#ClassificationVersionSoftware NameAuthor
1data analysisNMRPipeyear 2000
2structure solutionX-PLOR3.851
3refinementX-PLOR3.851