1SYZ
Solution structure of the S. Cerevisiae U6 intramolecular stem loop (ISL) RNA at pH 5.7
SOLUTION NMR
NMR Experiment | ||||||||
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Experiment | Type | Sample Contents | Solvent | Ionic Strength | pH | Pressure | Temperature (K) | Spectrometer |
1 | 2D NOESY | 0.8-1.2 mM RNA, 50 mM NaCl, pH 5.7 | H2O | 50 mM NaCl | 5.7 | Ambient | 285 | |
2 | 2D NOESY | 0.8-1.2 mM RNA, 50 mM NaCl, pH 5.7 | D2O | 50 mM NaCl | 5.7 | Ambient | 303 | |
3 | 2D TOCSY | 0.8-1.2 mM RNA, 50 mM NaCl, pH 5.7 | D2O | 50 mM NaCl | 5.7 | Ambient | 303 | |
4 | 1H-13C HSQC | 0.8-1.2 mM RNA, 50 mM NaCl, pH 5.7 | D2O | 50 mM NaCl | 5.7 | Ambient | 303 |
NMR Spectrometer Information | |||
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Spectrometer | Manufacturer | Model | Field Strength |
1 | Bruker | DMX | 750 |
2 | Bruker | DMX | 600 |
3 | Bruker | DMX |
NMR Refinement | ||
---|---|---|
Method | Details | Software |
XPLOR-NIH 2.0.6. | Method allowed for the incorporation of 29 residual dipolar couplings into the structure refinement process. | CNS |
NMR Ensemble Information | |
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Conformer Selection Criteria | structures with the lowest energy |
Conformers Calculated Total Number | 100 |
Conformers Submitted Total Number | 14 |
Representative Model | 1 (lowest energy) |
Additional NMR Experimental Information | |
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Details | 13C, 15N Istopically labelled RNA |
Computation: NMR Software | ||||
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# | Classification | Version | Software Name | Author |
1 | structure solution | CNS | 1.1 | Brunger, A. et al. |
2 | refinement | XPLORNIH | 2.0.6. | Clore, G.M. et. al. |
3 | collection | X-PLOR | 2.6 | Bruker Biospin |
4 | data analysis | Sparky | 3.0 | Goddard, T.D. et. al. |