1SP1
NMR STRUCTURE OF A ZINC FINGER DOMAIN FROM TRANSCRIPTION FACTOR SP1F3, MINIMIZED AVERAGE STRUCTURE
SOLUTION NMR
| NMR Experiment | ||||||||
|---|---|---|---|---|---|---|---|---|
| Experiment | Type | Sample Contents | Solvent | Ionic Strength | pH | Pressure | Temperature (K) | Spectrometer |
| 1 | NOESY | 5.9 | 278 | |||||
| 2 | COSY | 5.9 | 278 | |||||
| 3 | TOCSY | 5.9 | 278 | |||||
| NMR Spectrometer Information | |||
|---|---|---|---|
| Spectrometer | Manufacturer | Model | Field Strength |
| 1 | Bruker | AM500 | 500 |
| NMR Refinement | ||
|---|---|---|
| Method | Details | Software |
| DISTANCE GEOMETRY, SIMULATED ANNEALING | MULTIPLE ROUNDS OF SIMULATED ANNEALING REFINEMENT PROTOCOL OF X-PLOR | X-PLOR |
| NMR Ensemble Information | |
|---|---|
| Conformer Selection Criteria | MEAN STRUCTURE |
| Conformers Calculated Total Number | 100 |
| Conformers Submitted Total Number | 1 |
| Computation: NMR Software | ||||
|---|---|---|---|---|
| # | Classification | Version | Software Name | Author |
| 1 | refinement | X-PLOR | BRUNGER | |
| 2 | structure solution | Felix | ||
| 3 | structure solution | XPLOR | ||
