1SMG

CALCIUM-BOUND E41A MUTANT OF THE N-DOMAIN OF CHICKEN TROPONIN C, NMR, 40 STRUCTURES

SOLUTION NMR

NMR Experiment
Experiment	Type	Sample Contents	Solvent	Ionic Strength	pH	Pressure	Temperature (K)	Spectrometer
1	15N-HSQC				6.7		303.15
2	13C-CT-HSQC				6.7		303.15
3	HNCACB				6.7		303.15
4	CBCA(CO)NNH				6.7		303.15
5	15N-EDITED-NOESY				6.7		303.15
6	13C-EDITED-NOESY				6.7		303.15
7	2D-NOESY				6.7		303.15
8	15N-EDITED TOCSY				6.7		303.15
9	HCCHTOCSY				6.7		303.15
10	2D-COSY				6.7		303.15
11	HNHA				6.7		303.15

NMR Spectrometer Information
Spectrometer	Manufacturer	Model	Field Strength
1	Varian	UNITY-600	600

NMR Refinement
Method	Details	Software
SA.INP	REFINEMENT DETAILS CAN BE FOUND IN THE JRNL CITATION ABOVE.	X-PLOR

NMR Ensemble Information
Conformer Selection Criteria	LOWEST TOTAL ENERGY
Conformers Calculated Total Number	50
Conformers Submitted Total Number	40

Computation: NMR Software
#	Classification	Version	Software Name	Author
1	refinement	X-PLOR	3.1	BRUNGER
2	structure solution	X-PLOR	3.1

RCSB PDB Core Operations are funded by the U.S. National Science Foundation (DBI-2321666), the US Department of Energy (DE-SC0019749), and the National Cancer Institute, National Institute of Allergy and Infectious Diseases, and National Institute of General Medical Sciences of the National Institutes of Health under grant R01GM133198.