1S7E

Solution structure of HNF-6


SOLUTION NMR
NMR Experiment
ExperimentTypeSample ContentsSolventIonic StrengthpHPressureTemperature (K)Spectrometer
13D_15N-separated_NOESY1mM HNF-6 U-15N,13C, 50mM phosphate buffer NA, 90% H2O, 10% D2O90% H2O/10% D2O6.5295
23D HNCA1mM HNF-6 U-15N,13C, 50mM phosphate buffer NA, 90% H2O, 10% D2O90% H2O/10% D2O6.5295
32D 15N HSQC1mM HNF-6 U-15N, 50mM phosphate buffer NA, 90% H2O, 10% D2O90% H2O/10% D2O
43D_13C-separated_NOESY1mM HNF-6 U-15N,13C 2H, 50mM phosphate buffer NA, 90% H2O, 10% D2O90% H2O/10% D2O
52D NOESY1mM HNF-6 U-15N,13C 2H, 50mM phosphate buffer NA, 90% H2O, 10% D2O90% H2O/10% D2O
63D HNCACB, CBCA(CO)NH1mM HNF-6 U-13C, 50mM phosphate buffer NA100% D2O
NMR Spectrometer Information
SpectrometerManufacturerModelField Strength
1BrukerDRX600
2BrukerDMX750
3VarianINOVA800
NMR Refinement
MethodDetailsSoftware
torsion angle dynamicsDYANA
NMR Ensemble Information
Conformer Selection Criteriatarget function
Conformers Calculated Total Number100
Conformers Submitted Total Number20
Representative Model1 (lowest target function)
Additional NMR Experimental Information
DetailsThis structure was determined using standard 2D and 3D homonuclear and heteronuclear techniques
Computation: NMR Software
#ClassificationVersionSoftware NameAuthor
1structure solutionDYANA1.5Guentert
2refinementDYANA1.5Guentert