1RZW

The Solution Structure of the Archaeglobus fulgidis protein AF2095. Northeast Structural Genomics Consortium target GR4


SOLUTION NMR
NMR Experiment
ExperimentTypeSample ContentsSolventIonic StrengthpHPressureTemperature (K)Spectrometer
13D_13C-separated_NOESY20 mM MES, 100 mM NaCl, 5 mM CaCl2, 10 mM DTT, 0.02% NaN3, 5% D2O pH 6.595% H2O/5% D2O20 mM MES, 100 mM NaCl, 5 mM CaCl2, 10 mM DTT, 0.02% NaN36.5ambient313
23D_15N-separated_NOESY20 mM MES, 100 mM NaCl, 5 mM CaCl2, 10 mM DTT, 0.02% NaN3, 5% D2O pH 6.595% H2O/5% D2O20 mM MES, 100 mM NaCl, 5 mM CaCl2, 10 mM DTT, 0.02% NaN36.5ambient313
3HNHA20 mM MES, 100 mM NaCl, 5 mM CaCl2, 10 mM DTT, 0.02% NaN3, 5% D2O pH 6.595% H2O/5% D2O20 mM MES, 100 mM NaCl, 5 mM CaCl2, 10 mM DTT, 0.02% NaN36.5ambient313
4triple-resonance experiments for backbone/sidechain assignments20 mM MES, 100 mM NaCl, 5 mM CaCl2, 10 mM DTT, 0.02% NaN3, 5% D2O pH 6.595% H2O/5% D2O20 mM MES, 100 mM NaCl, 5 mM CaCl2, 10 mM DTT, 0.02% NaN36.5ambient313
NMR Spectrometer Information
SpectrometerManufacturerModelField Strength
1BrukerAVANCE600
2BrukerAVANCE750
3BrukerAVANCE500
NMR Refinement
MethodDetailsSoftware
distance geometry simulated annealing molecular dynamicsNMRPipe
NMR Ensemble Information
Conformer Selection Criteria
Conformers Calculated Total Number
Conformers Submitted Total Number1
Computation: NMR Software
#ClassificationVersionSoftware NameAuthor
1processingNMRPipe2.2Delaglio
2structure solutionXPLOR-NIH2.9.1Schwieters, Kuszewski, Tjandra, Clore, Brunger
3data analysisPIPP4.3.5Garrett
4data analysisAutoAssign1.11.1Zimmerman, Moseley, Kiriyevs,Kulikowski,Montelione
5refinementAutoStructure2.0bHuang, Montelione