1RQ6

Solution structure of ribosomal protein S17E from Methanobacterium Thermoautotrophicum, Northeast Structural Genomics Consortium Target TT802 / Ontario Center for Structural Proteomics Target Mth0803


SOLUTION NMR
NMR Experiment
ExperimentTypeSample ContentsSolventIonic StrengthpHPressureTemperature (K)Spectrometer
13D_15N-separated_NOESY1 mM mth0803, U-15N,13C; 450 mM NaCl, 25 mM Na2PO4, 1mM Benzamidine, 1xinhibitor cooktail, 0.01% NaN3, 95% H2O, 5% D2O95% H2O/5% D2O450 mM NaCl, 25 mM Phosphate buffer6.5ambient298
23D_13C-separated_NOESY1 mM mth0803, U-15N,13C; 450 mM NaCl, 25 mM Na2PO4, 1mM Benzamidine, 1xinhibitor cooktail, 0.01% NaN3, 95% H2O, 5% D2O95% H2O/5% D2O450 mM NaCl, 25 mM Phosphate buffer6.5ambient298
34D_13C-separated_NOESY1 mM mth0803, U-15N,13C; 450 mM NaCl, 25 mM Na2PO4, 1mM Benzamidine, 1xinhibitor cooktail, 0.01% NaN3, 95% H2O, 5% D2O95% H2O/5% D2O450 mM NaCl, 25 mM Phosphate buffer6.5ambient298
4HNHA1 mM mth0803, U-15N,13C; 450 mM NaCl, 25 mM Na2PO4, 1mM Benzamidine, 1xinhibitor cooktail, 0.01% NaN3, 95% H2O, 5% D2O95% H2O/5% D2O450 mM NaCl, 25 mM Phosphate buffer6.5ambient298
NMR Spectrometer Information
SpectrometerManufacturerModelField Strength
1VarianINOVA600
NMR Refinement
MethodDetailsSoftware
torsion angle dyanmics1143 NOE-derived distance constraints and 159 angle constraints (PHI and PSI)NMRPipe
NMR Ensemble Information
Conformer Selection Criteriastructures with the least restraint violations,target function
Conformers Calculated Total Number112
Conformers Submitted Total Number15
Representative Model1 (lowest energy)
Computation: NMR Software
#ClassificationVersionSoftware NameAuthor
1processingNMRPipe2.1Delaglio,F.
2structure solutionAutoStructure2.0Huang, Y.J., Montelione, G.T.
3refinementDYANA1.5Gunter,P.
4refinementCNS1.0Brunger, A.T. et al.