1QKL
hRPABC14.4, essential subunit of human RNA polymerases I, II and III
SOLUTION NMR
| NMR Experiment | ||||||||
|---|---|---|---|---|---|---|---|---|
| Experiment | Type | Sample Contents | Solvent | Ionic Strength | pH | Pressure | Temperature (K) | Spectrometer |
| 1 | VARIOUS TRIPLE RESONANCE & NOES EXPERIMENTS | 90% WATER/10% D2O | 7.5 | 1 atm | 298 | |||
| NMR Spectrometer Information | |||
|---|---|---|---|
| Spectrometer | Manufacturer | Model | Field Strength |
| 1 | Varian | UNITY | 500 |
| 2 | Varian | INOVA | 500 |
| 3 | Varian | INOVA | 750 |
| 4 | Bruker | AMX | 600 |
| NMR Refinement | ||
|---|---|---|
| Method | Details | Software |
| DISTANCE GEOMETRY AND SIMULATED ANNEALING | RESIDUES 801-832 ARE DISORDERED | X-PLOR |
| NMR Ensemble Information | |
|---|---|
| Conformer Selection Criteria | LEAST RESTRAINT VIOLATIONS |
| Conformers Calculated Total Number | 75 |
| Conformers Submitted Total Number | 22 |
| Representative Model | 1 (n/a) |
| Additional NMR Experimental Information | |
|---|---|
| Details | THE STRUCTURE WAS DETERMINED USING TRIPLE RESONANCE EXPERIMENTS ON 13C AND 15N-LABELED HRPABC14.4 |
| Computation: NMR Software | ||||
|---|---|---|---|---|
| # | Classification | Version | Software Name | Author |
| 1 | refinement | X-PLOR | 3.851 | BRUNGER |
| 2 | structure solution | FELIX | ||
| 3 | structure solution | XEASY | ||
| 4 | structure solution | X-PLOR | ||
