1QC8

NMR STRUCTURE OF TAU EXON 10 SPLICING REGULATORY ELEMENT RNA

SOLUTION NMR

NMR Experiment
Experiment	Type	Sample Contents	Solvent	Ionic Strength	pH	Pressure	Temperature (K)	Spectrometer
1	2D NOESY	1MM RNA, 10MM NAPHOSPHATE BUFFER PH 6		10mM NAPHOSPHATE BUFFER PH 6	6		300
2	3D_13C-SEPARATED_NOESY	1MM RNA, U 15N,13C, 10MM NAPHOSPHATE BUFFER PH 6		10mM NAPHOSPHATE BUFFER PH 6	6		300
3	DQF-COSY	1MM RNA, 10MM NAPHOSPHATE BUFFER PH 6		10mM NAPHOSPHATE BUFFER PH 6	6		300
4	2D TOCSY	1MM RNA, 10MM NAPHOSPHATE BUFFER PH 6		10mM NAPHOSPHATE BUFFER PH 6	6		300

NMR Spectrometer Information
Spectrometer	Manufacturer	Model	Field Strength
1	Bruker	DMX	600

NMR Refinement
Method	Details	Software
COMPLETELY RANDOM STARTING COORDINATES X-PLOR BASED SIMULATED ANNEALING PROTOCOL	NOE DISTANCE CONSTRAINTS: 564, INTRARESIDUE: 331, SEQUENTIAL: 181, LONG AND MEDIUM RANGE: 52, HYDROGEN BONDING AND PLANARITY CONSTRAINTS: 90, DIHEDRAL CONSTRAINTS: 124, TOTAL EXPERIMENTAL CONSTRAINTS: 778, AVERAGE NUMBER PER NUCLEOTIDE: 31.1	UXNMR

NMR Ensemble Information
Conformer Selection Criteria	structures with the lowest energy
Conformers Calculated Total Number	50
Conformers Submitted Total Number	29

Computation: NMR Software
#	Classification	Version	Software Name	Author
1	collection	UXNMR
2	processing	Felix	2.10
3	data analysis	Felix	97
4	refinement	X-PLOR	3.851	Brunger

RCSB PDB Core Operations are funded by the U.S. National Science Foundation (DBI-2321666), the US Department of Energy (DE-SC0019749), and the National Cancer Institute, National Institute of Allergy and Infectious Diseases, and National Institute of General Medical Sciences of the National Institutes of Health under grant R01GM133198.