1Q60
Solution Structure of RSGI RUH-004, a GTF2I domain in Mouse cDNA
SOLUTION NMR
| NMR Experiment | ||||||||
|---|---|---|---|---|---|---|---|---|
| Experiment | Type | Sample Contents | Solvent | Ionic Strength | pH | Pressure | Temperature (K) | Spectrometer |
| 1 | 3D_13C-separated_NOESY | 1.4mM GTF2I domain U-15N, 13C; 20mM phosphate buffer (pH 7.2); 100mM NaCl; 1mM DTT; 90% H2O, 10% D2O | 90% H2O/10% D2O | 120mM NaCl | 7.2 | ambient | 298 | |
| 2 | 3D_15N-separated_NOESY | 1.4mM GTF2I domain U-15N, 13C; 20mM phosphate buffer (pH 7.2); 100mM NaCl; 1mM DTT; 90% H2O, 10% D2O | 90% H2O/10% D2O | 120mM NaCl | 7.2 | ambient | 298 | |
| NMR Spectrometer Information | |||
|---|---|---|---|
| Spectrometer | Manufacturer | Model | Field Strength |
| 1 | Varian | INOVA | 800 |
| NMR Refinement | ||
|---|---|---|
| Method | Details | Software |
| torsion angle dynamics | VNMR | |
| NMR Ensemble Information | |
|---|---|
| Conformer Selection Criteria | structures with the least restraint violations, structures with the lowest energy, target function |
| Conformers Calculated Total Number | 100 |
| Conformers Submitted Total Number | 20 |
| Representative Model | 1 (lowest energy) |
| Computation: NMR Software | ||||
|---|---|---|---|---|
| # | Classification | Version | Software Name | Author |
| 1 | collection | VNMR | 6.1C | Varian |
| 2 | processing | NMRPipe | 20020425 | Delaglio, F. |
| 3 | data analysis | NMRView | 5.0.4 | Johnson, B. A. |
| 4 | data analysis | KUJIRA | 0.816 | Kobayashi, N. |
| 5 | structure solution | CYANA | 1.0.7 | Guentert, P. |
| 6 | refinement | CYANA | 1.0.7 | Guentert, P. |
