1Q59

Solution Structure of the BHRF1 Protein From Epstein-Barr Virus, a Homolog of Human Bcl-2

SOLUTION NMR

NMR Experiment
Experiment	Type	Sample Contents	Solvent	Ionic Strength	pH	Pressure	Temperature (K)	Spectrometer
1	3D_13C-separated_NOESY	U-15N,13C 0.5-1.0 mM BHRF1-EBV-BCL2 , containing 20 mM Tris (pH 7.5) and 2 mM 2H-dithiothreitol	90% H2O/10%2H2O	20mM	7.5	ambient	303
2	3D_15N-separated_NOESY	U-15N,13C 0.5-1.0 mM BHRF1-EBV-BCL2 , containing 20 mM Tris (pH 7.5) and 2 mM 2H-dithiothreitol	90% H2O/10%2H2O	20mM	7.5	ambient	303

NMR Spectrometer Information
Spectrometer	Manufacturer	Model	Field Strength
1	Bruker	DRX	500
2	Bruker	DRX	800
3	Bruker	DRX	600

NMR Refinement
Method	Details	Software
BHRF1 structures were calculated using a simulated annealing protocol with the program CNX		CNS

NMR Ensemble Information
Conformer Selection Criteria
Conformers Calculated Total Number
Conformers Submitted Total Number	1
Representative Model	1 (minimized average structure)

Computation: NMR Software
#	Classification	Version	Software Name	Author
1	refinement	CNS	2000

RCSB PDB Core Operations are funded by the U.S. National Science Foundation (DBI-2321666), the US Department of Energy (DE-SC0019749), and the National Cancer Institute, National Institute of Allergy and Infectious Diseases, and National Institute of General Medical Sciences of the National Institutes of Health under grant R01GM133198.