1PSY
STRUCTURE OF RicC3, NMR, 20 STRUCTURES
SOLUTION NMR
| NMR Experiment | ||||||||
|---|---|---|---|---|---|---|---|---|
| Experiment | Type | Sample Contents | Solvent | Ionic Strength | pH | Pressure | Temperature (K) | Spectrometer |
| 1 | 3D_15N-separated_NOESY | 2mM RicC3 15N; 10mM sodium phosphate buffer ;95% H2O, 5% D2O | 3.5 | 308 | ||||
| 2 | 2D NOESY | 2mM RicC3 10mM sodium phosphate buffer ;95% H2O, 5% D2O | 3.5 | 308 | ||||
| 3 | 2D NOESY | 2mM RicC3 10mM sodium phosphate buffer ;100% D2O | 3.5 | 308 | ||||
| NMR Spectrometer Information | |||
|---|---|---|---|
| Spectrometer | Manufacturer | Model | Field Strength |
| 1 | Bruker | DMX | 600 |
| NMR Refinement | ||
|---|---|---|
| Method | Details | Software |
| simulated annealing, torsion angle dynamics | NMRPipe | |
| NMR Ensemble Information | |
|---|---|
| Conformer Selection Criteria | |
| Conformers Calculated Total Number | |
| Conformers Submitted Total Number | 20 |
| Computation: NMR Software | ||||
|---|---|---|---|---|
| # | Classification | Version | Software Name | Author |
| 1 | processing | NMRPipe | Delaglio | |
| 2 | data analysis | NMRView | 5.0.4 | Jhonson |
| 3 | structure solution | DYANA | 1.5 | Guentert |
| 4 | refinement | Amber | 7 | Case |
