NMR Experiment |
---|
Experiment | Type | Sample Contents | Solvent | Ionic Strength | pH | Pressure | Temperature (K) | Spectrometer |
---|
1 | 3D_15N-separated_NOESY | 1mM MutT, 1.3mM 8-oxo-dGMP, 15mM MgCl2, 4mM d11-Tris-HCl, pH 7.5, 21mM NaCl, 0.34mM NaN3. | 90% H2O/10% D2O | 21mM NaCl | 7.5 | ambient | 295 | |
2 | 2D NOESY | 1mM MutT, 1.3mM 8-oxo-dGMP, 15mM MgCl2, 4mM d11-Tris-HCl, pH 7.5, 21mM NaCl, 0.34mM NaN3. | 90% H2O/10% D2O | 21mM NaCl | 7.5 | ambient | 295 | |
3 | 2D TOCSY | 1mM MutT, 1.3mM 8-oxo-dGMP, 15mM MgCl2, 4mM d11-Tris-HCl, pH 7.5, 21mM NaCl, 0.34mM NaN3. | 90% H2O/10% D2O | 21mM NaCl | 7.5 | ambient | 295 | |
4 | 3D_13C-separated_NOESY | 1mM MutT, 1.3mM 8-oxo-dGMP, 15mM MgCl2, 4mM d11-Tris-HCl, pH 7.5, 21mM NaCl, 0.34mM NaN3. | 90% H2O/10% D2O | 21mM NaCl | 7.5 | ambient | 295 | |
5 | 15N-1H IPAP-HSQC | 1mM MutT, 1.3mM 8-oxo-dGMP, 15mM MgCl2, 4mM d11-Tris-HCl, pH 7.5, 21mM NaCl, 0.34mM NaN3. | 90% H2O, 10% D2O.
3% (w/v) bicelles (C8E5/octanol ratio 0.87) | 21mM NaCl | 7.5 | ambient | 295 | |
6 | 12C/13C-filtered-NOESY-HSQC | 1mM MutT, 1.3mM 8-oxo-dGMP, 15mM MgCl2, 4mM d11-Tris-HCl, pH 7.5, 21mM NaCl, 0.34mM NaN3. | 90% H2O/10% D2O | 21mM NaCl | 7.5 | ambient | 295 | |
7 | HNCA | 1mM MutT, 1.3mM 8-oxo-dGMP, 15mM MgCl2, 4mM d11-Tris-HCl, pH 7.5, 21mM NaCl, 0.34mM NaN3. | 90% H2O/10% D2O | 21mM NaCl | 7.5 | ambient | 295 | |
8 | NHCACB | 1mM MutT, 1.3mM 8-oxo-dGMP, 15mM MgCl2, 4mM d11-Tris-HCl, pH 7.5, 21mM NaCl, 0.34mM NaN3. | 90% H2O/10% D2O | 21mM NaCl | 7.5 | ambient | 295 | |