Experiment: 1P5N

SOLUTION NMR

NMR Experiment
Experiment	Type	Sample Contents	Solvent	Ionic Strength	pH	Pressure	Temperature (K)	Spectrometer
1	2D S-NOESY	1-2mM HCV IRES domain IIb RNA 10mM phosphate buffer	96% D2O, 4% H2O	10mM posphate buffer	6.40	ambient	288
2	2D NOESY	1-2mM HCV IRES domain IIb RNA 10mM phosphate buffer	100% D2O	10mM posphate buffer	6.40	ambient	298
3	DQF-COSY	1-2mM HCV IRES domain IIb RNA 10mM phosphate buffer	100% D2O	10mM posphate buffer	6.40	ambient	298
4	2D HP-COSY	1-2mM HCV IRES domain IIb RNA 10mM phosphate buffer	100% D2O	10mM posphate buffer	6.40	ambient	298
5	3D_13C-separated_NOESY	1-2mM HCV IRES domain IIb RNA U-15N,13C 10mM phosphate buffer	100% D2O	10mM posphate buffer	6.40	ambient	298
6	3D HCP	1-2mM HCV IRES domain IIb RNA U-15N,13C 10mM phosphate buffer	100% D2O	10mM posphate buffer	6.40	ambient	298
7	3D HMQC TOCSY	1-2mM HCV IRES domain IIb RNA U-15N,13C 10mM phosphate buffer	96% D2O, 4% H2O	10mM posphate buffer	6.40	ambient	298

NMR Spectrometer Information
Spectrometer	Manufacturer	Model	Field Strength
1	Varian	INOVA	500
2	Varian	INOVA	800

NMR Refinement
Method	Details	Software
see reference above	see reference above	VNMR

NMR Ensemble Information
Conformer Selection Criteria	lowest restraint violation and total energy
Conformers Calculated Total Number	100
Conformers Submitted Total Number	20
Representative Model	1 (minimized average structure)

Additional NMR Experimental Information
Details	The structure was solved using triple resonance, multidimensional NMR spectroscopy and TROSY-type experiments to measure residual dipolar couplings

Computation: NMR Software
#	Classification	Version	Software Name	Author
1	processing	VNMR	6.1C	Varian
2	data analysis	Sparky	3	Goddard, T.D. & Kneller D.G.
3	refinement	X-PLOR	3.1	Brunger, A.T.
4	refinement	CNS	1.1	Brunger, A.T.
5	refinement	PALES	1	Zweckstetter, M. & Bax, A.

RCSB PDB Core Operations are funded by the National Science Foundation (DBI-1832184), the US Department of Energy (DE-SC0019749), and the National Cancer Institute, National Institute of Allergy and Infectious Diseases, and National Institute of General Medical Sciences of the National Institutes of Health under grant R01GM133198.