1P1T
NMR Structure of the N-terminal RRM domain of Cleavage stimulation factor 64 KDa subunit
SOLUTION NMR
| NMR Experiment | ||||||||
|---|---|---|---|---|---|---|---|---|
| Experiment | Type | Sample Contents | Solvent | Ionic Strength | pH | Pressure | Temperature (K) | Spectrometer |
| 1 | 2D NOESY | 1mM CstF U-15N, 20mM phosphate buffer | 93% H2O/7% D2O | 20mM | 6.0 | ambient | 300 | |
| 2 | 3D_15N-separated_NOESY | 1mM CstF U-15N, 20mM phosphate buffer | 93% H2O/7% D2O | 20mM | 6.0 | ambient | 300 | |
| 3 | 2D NOESY | 1mM CstF , 20mM phosphate buffer | 100% D2O | 20mM | 6.0 | ambient | 300 | |
| 4 | 15N-HSQC | 1mM CstF U-15N, 20mM phosphate buffer | 93% H2O/7% D2O | 20mM | 6.0 | ambient | 300 | |
| 5 | 13C-HSQC | 1mM CstF U-15N,13C, 20mM phosphate buffer | 93% H2O/7% D2O | 20mM | 6.0 | ambient | 300 | |
| 6 | HNCA | 1mM CstF U-15N,13C, 20mM phosphate buffer | 93% H2O/7% D2O | 20mM | 6.0 | ambient | 300 | |
| 7 | HNCO | 1mM CstF U-15N,13C, 20mM phosphate buffer | 93% H2O/7% D2O | 20mM | 6.0 | ambient | 300 | |
| 8 | CBCA(CO)NH | 1mM CstF U-15N,13C, 20mM phosphate buffer | 93% H2O/7% D2O | 20mM | 6.0 | ambient | 300 | |
| 9 | HCCH-COSY | 1mM CstF U-15N,13C, 20mM phosphate buffer | 93% H2O/7% D2O | 20mM | 6.0 | ambient | 300 | |
| NMR Spectrometer Information | |||
|---|---|---|---|
| Spectrometer | Manufacturer | Model | Field Strength |
| 1 | Bruker | DRX | 500 |
| 2 | Bruker | DMX | 600 |
| 3 | Bruker | AVANCE | 800 |
| NMR Refinement | ||
|---|---|---|
| Method | Details | Software |
| simulated annealing torsion angle dynamics | The structures are based on a total of 1486 NOE-derived distance contraints and 115 dihedral angle restrains for phi and psi backbone angles obtained from the analysis of the 13C chemical shifts. | NMRPipe |
| NMR Ensemble Information | |
|---|---|
| Conformer Selection Criteria | The submitted conformer models are the 20 structures with the lowest energy |
| Conformers Calculated Total Number | 40 |
| Conformers Submitted Total Number | 20 |
| Representative Model | 1 (fewest violations) |
| Additional NMR Experimental Information | |
|---|---|
| Details | The structure was determined using triple-resoncance NMR spectroscopy |
| Computation: NMR Software | ||||
|---|---|---|---|---|
| # | Classification | Version | Software Name | Author |
| 1 | processing | NMRPipe | 2001 | Delaglio, Grzesiek, Vuister, Pfeifer, Bax |
| 2 | structure solution | DYANA | 1.5 | Guntert, Mumenthaler, Herrmann |
| 3 | data analysis | TALOS | 2001 | Cornilescu, Delaglio, Bax |
| 4 | refinement | DYANA | 1.5 | Guntert, Mumenthaler, Herrmann |
