1OZS
C-domain of human cardiac troponin C in complex with the inhibitory region of human cardiac troponin I
SOLUTION NMR
NMR Experiment | ||||||||
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Experiment | Type | Sample Contents | Solvent | Ionic Strength | pH | Pressure | Temperature (K) | Spectrometer |
1 | 3D_13C-separated_NOESY | 13C/15N-cCTnC in complex with 13C/15N-cIp | 100 mM KCl, 10 mM Imidazole, 20mM CaCl2, 1mM DSS, pH 6.7 | 0.1 | 6.7 | 1 atm | 303 | |
2 | HNHA | 13C/15N-cCTnC in complex with 13C/15N-cIp | 100 mM KCl, 10 mM Imidazole, 20mM CaCl2, 1mM DSS, pH 6.7 | 0.1 | 6.7 | 1 atm | 303 | |
3 | 3D_15N-separated_NOESY | 13C/15N-cCTnC in complex with 13C/15N-cIp | 100 mM KCl, 10 mM Imidazole, 20mM CaCl2, 1mM DSS, pH 6.7 | 0.1 | 6.7 | 1 atm | 303 | |
4 | 3D_13C-TOCSY | 13C/15N-cCTnC in complex with 13C/15N-cIp | 100 mM KCl, 10 mM Imidazole, 20mM CaCl2, 1mM DSS, pH 6.7 | 0.1 | 6.7 | 1 atm | 303 | |
5 | 3D_15N-TOCSY | 13C/15N-cCTnC in complex with 13C/15N-cIp | 100 mM KCl, 10 mM Imidazole, 20mM CaCl2, 1mM DSS, pH 6.7 | 0.1 | 6.7 | 1 atm | 303 |
NMR Spectrometer Information | |||
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Spectrometer | Manufacturer | Model | Field Strength |
1 | Varian | INOVA | 800 |
2 | Varian | INOVA | 500 |
3 | Varian | UNITY | 600 |
NMR Refinement | ||
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Method | Details | Software |
100 structures calculated using CNS simulated annealing protocol starting from extended structures | VNMR |
NMR Ensemble Information | |
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Conformer Selection Criteria | structures with the lowest energy |
Conformers Calculated Total Number | 100 |
Conformers Submitted Total Number | 30 |
Computation: NMR Software | ||||
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# | Classification | Version | Software Name | Author |
1 | collection | VNMR | 6.1b | varian inc. |
2 | structure solution | CNS | 1.1 | Brunger |
3 | processing | NMRPipe | 1 | Delaglio |
4 | refinement | CNS | 1.1 | Brunger |