1NTT

5'(dCPCPUPCPCPUPUP)3':(rAGGAGGAAA)5', where P=propynyl


SOLUTION NMR
NMR Experiment
ExperimentTypeSample ContentsSolventIonic StrengthpHPressureTemperature (K)Spectrometer
12D NOESY~2.5 mM hybrid, 80 mM NaCl, 10 mM phosphate, 0.5 mM EDTA99.996% D2O80 mM NaCl7.0ambient303
NMR Spectrometer Information
SpectrometerManufacturerModelField Strength
1VarianINOVA500
NMR Refinement
MethodDetailsSoftware
simulated annealing, energy minimizationThe structure is based on a total of 160 interproton distance restraints, 18 hydrogen bond restraints, and 59 dihedral angle restraints.VNMR
NMR Ensemble Information
Conformer Selection Criteria
Conformers Calculated Total Number
Conformers Submitted Total Number1
Representative Model1 (minimized average structure)
Computation: NMR Software
#ClassificationVersionSoftware NameAuthor
1collectionVNMR5.2Varian
2data analysisFelix2000MSI
3structure solutionDiscover95.0MSI
4refinementDiscover95.0MSI