1N6Z

Solution NMR Structure of Protein YML108W from Saccharomyces cerevisiae. A novel member of the split bab fold. Northeast Structural Genomics Consortium Target YT601.

SOLUTION NMR

NMR Experiment
Experiment	Type	Sample Contents	Solvent	Ionic Strength	pH	Pressure	Temperature (K)	Spectrometer
1	3D_13C-separated_NOESY	1 mM, U-15N,13C 25 mM sodium phosphate (pH=6.5), 450 mM NaCl, 1 mM DTT	95% H20/5% D2O	900E-3	6.5	AMBIENT	298
2	3D_15N-separated_NOESY	1 mM, U-15N,13C 25 mM sodium phosphate (pH=6.5), 450 mM NaCl, 1 mM DTT	95% H20/5% D2O	900E-3	6.5	AMBIENT	298

NMR Spectrometer Information
Spectrometer	Manufacturer	Model	Field Strength
1	Varian	UNITY	600

NMR Refinement
Method	Details	Software
torsion angle dynamics		NMRPipe

NMR Ensemble Information
Conformer Selection Criteria	structures with the lowest energy
Conformers Calculated Total Number	200
Conformers Submitted Total Number	10
Representative Model	1 (lowest energy)

Additional NMR Experimental Information
Details	THIS STRUCTURE WAS DETERMINED USING STANDARD 3D HETERONUECLEAR NMR TECHNIQUES

Computation: NMR Software
#	Classification	Version	Software Name	Author
1	processing	NMRPipe	2.1	DELAGLIO ET AL.
2	structure solution	DYANA	1.5	GUNTERT ET AL.
3	data analysis	XEASY	1.3.14	BARTELS ET AL.
4	structure solution	CYANA	1.0.5	HERRMANN ET AL.
5	refinement	DYANA	1.5	GUNTERT ET AL.

RCSB PDB Core Operations are funded by the National Science Foundation (DBI-1832184), the US Department of Energy (DE-SC0019749), and the National Cancer Institute, National Institute of Allergy and Infectious Diseases, and National Institute of General Medical Sciences of the National Institutes of Health under grant R01GM133198.