1MV0

NMR STRUCTURE OF THE TUMOR SUPPRESSOR BIN1: ALTERNATIVE SPLICING IN MELANOMA AND INTERACTION WITH C-MYC


SOLUTION NMR
NMR Experiment
ExperimentTypeSample ContentsSolventIonic StrengthpHPressureTemperature (K)Spectrometer
13D_13C-separated_NOESY1.4 mM Bin1(402-482)/c-Myc(55-68) U-15N, 13C, 25 mM sodium phosphate, 150 mM NaCl, 1 mM DTT, 95% H2O, 5% D2O pH=6.595% H2O/5% D2O300e-36.5ambient298
23D_15N-separated_NOESY1.4 mM Bin1(402-482)/c-Myc(55-68) U-15N, 13C, 25 mM sodium phosphate, 150 mM NaCl, 1 mM DTT, 95% H2O, 5% D2O pH=6.595% H2O/5% D2O300e-36.5ambient298
NMR Spectrometer Information
SpectrometerManufacturerModelField Strength
1VarianUNITY600
NMR Refinement
MethodDetailsSoftware
torsion angle dynamicsNMRPipe
NMR Ensemble Information
Conformer Selection Criteriastructures with the lowest energy
Conformers Calculated Total Number200
Conformers Submitted Total Number20
Representative Model1 (lowest energy)
Additional NMR Experimental Information
DetailsTHIS STRUCTURE WAS DETERMINED USING STANDARD 3D HETERONUCLEAR NMR TECHNIQUES
Computation: NMR Software
#ClassificationVersionSoftware NameAuthor
1processingNMRPipe2.1DELAGLIO ET AL.
2structure solutionDYANA1.5GUNTERT ET AL.
3data analysisXEASY1.3.13BARTELS ET AL.
4refinementDYANA1.5GUNTERT ET AL.