1MT4
Structure of 23S ribosomal RNA hairpin 35
SOLUTION NMR
| NMR Experiment | ||||||||
|---|---|---|---|---|---|---|---|---|
| Experiment | Type | Sample Contents | Solvent | Ionic Strength | pH | Pressure | Temperature (K) | Spectrometer |
| 1 | HSQC | 1mM RNA U-15N; 10mM phosphate buffer; 90% H2O, 10% D2O | 90% H2O/10% D2O | 10mM phosphate | 6.4 | ambient | 303 | |
| 2 | TOCSY, 3D NOESY-HMQC, 3D HCCH- TOCSY, HP COSY, HSQC, 3D HCP | 1mM RNA 15N-13C; 10mM phosphate buffer; 100% D2O | 100% D2O | |||||
| NMR Spectrometer Information | |||
|---|---|---|---|
| Spectrometer | Manufacturer | Model | Field Strength |
| 1 | Bruker | DRX | 800 |
| 2 | Bruker | DRX | 600 |
| NMR Refinement | ||
|---|---|---|
| Method | Details | Software |
| simulated annealing | XwinNMR | |
| NMR Ensemble Information | |
|---|---|
| Conformer Selection Criteria | structures with the lowest energy |
| Conformers Calculated Total Number | 115 |
| Conformers Submitted Total Number | 17 |
| Representative Model | 1 (lowest energy) |
| Computation: NMR Software | ||||
|---|---|---|---|---|
| # | Classification | Version | Software Name | Author |
| 1 | processing | XwinNMR | ||
| 2 | processing | AURELIA | ||
| 3 | structure solution | Discover | ||
| 4 | refinement | Discover | ||
