NMR Experiment |
---|
Experiment | Type | Sample Contents | Solvent | Ionic Strength | pH | Pressure | Temperature (K) | Spectrometer |
---|
1 | 3D 13C/15N-separated NOESY | 1.5 mM BAG4 BD U-15N 13C, 10 mM
potassium phosphate buffer pH 7.2,
100 mM KCl, 1 mM DTT, 1 mM EDTA | 90% H2O/10% D2O | 0.12 | 7.2 | ambient | 303 | |
2 | 3D_15N-separated_NOESY | 1.5 mM BAG4 BD U-15N, 10 mM
potassium phosphate buffer pH 7.2,
100 mM KCl, 1 mM DTT, 1 mM EDTA | 90% H2O/10% D2O | 0.12 | 7.2 | ambient | 303 | |
3 | 3D CBCA(CO)NH | 1.5 mM BAG4 BD U-15N 13C, 10 mM
potassium phosphate buffer pH 7.2,
100 mM KCl, 1 mM DTT, 1 mM EDTA | 90% H2O/10% D2O | 0.12 | 7.2 | ambient | 303 | |
4 | 3D HNCACB | 1.5 mM BAG4 BD U-15N 13C, 10 mM
potassium phosphate buffer pH 7.2,
100 mM KCl, 1 mM DTT, 1 mM EDTA | 90% H2O/10% D2O | 0.12 | 7.2 | ambient | 303 | |
5 | 3D H(CCO)NH | 1.5 mM BAG4 BD U-15N 13C, 10 mM
potassium phosphate buffer pH 7.2,
100 mM KCl, 1 mM DTT, 1 mM EDTA | 90% H2O/10% D2O | 0.12 | 7.2 | ambient | 303 | |
6 | 3D C(CO)NH | 1.5 mM BAG4 BD U-15N 13C, 10 mM
potassium phosphate buffer pH 7.2,
100 mM KCl, 1 mM DTT, 1 mM EDTA | 90% H2O/10% D2O | 0.12 | 7.2 | ambient | 303 | |
7 | 3D HNCO | 1.5 mM BAG4 BD U-15N 13C, 10 mM
potassium phosphate buffer pH 7.2,
100 mM KCl, 1 mM DTT, 1 mM EDTA | 90% H2O/10% D2O | 0.12 | 7.2 | ambient | 303 | |