1M11

structural model of human decay-accelerating factor bound to echovirus 7 from cryo-electron microscopy


ELECTRON MICROSCOPY
Sample
human decay-accelerating factor, HUMAN ECHOVIRUS 7 COAT PROTEINS
Specimen Preparation
Sample Aggregation StatePARTICLE
Vitrification Instrument
Cryogen Name
Sample Vitrification DetailsSAMPLES WERE PREPARED AS THIN LAYERS OF VITREOUS ICE AND MAINTAINED AT NEAR LIQUID NITROGEN TEMPERATURE IN THE ELECTRON MICROSCOPE ...SAMPLES WERE PREPARED AS THIN LAYERS OF VITREOUS ICE AND MAINTAINED AT NEAR LIQUID NITROGEN TEMPERATURE IN THE ELECTRON MICROSCOPE WITH A GATAN 626 CRYOTRANSFER HOLDER
3D Reconstruction
Reconstruction MethodSINGLE PARTICLE
Number of Particles
Reported Resolution (Å)16
Resolution Method
Other DetailsThe echovirus 7 structure is unknown, the model used here is from coxsackievirus B3 (1COV) and echovirus 1 (1EV1).The DAF receptor model is from 1g40. ...The echovirus 7 structure is unknown, the model used here is from coxsackievirus B3 (1COV) and echovirus 1 (1EV1).The DAF receptor model is from 1g40. Only CA coordinates are presented in the entry.
Refinement Type
Symmetry TypePOINT
Point SymmetryI
Map-Model Fitting and Refinement
Id1 (1G40, 1COV, 1EV1)
Refinement SpaceREAL
Refinement Protocol
Refinement Target
Overall B Value
Fitting Procedure
Details
Data Acquisition
Detector Type
Electron Dose (electrons/Å**2)16.6
Imaging Experiment1
Date of Experiment2001-09-10
Temperature (Kelvin)120
Microscope ModelFEI/PHILIPS CM300FEG/T
Minimum Defocus (nm)1800
Maximum Defocus (nm)4200
Minimum Tilt Angle (degrees)
Maximum Tilt Angle (degrees)
Nominal CS
Imaging ModeBRIGHT FIELD
Specimen Holder Model
Nominal Magnification45000
Calibrated Magnification
SourceFIELD EMISSION GUN
Acceleration Voltage (kV)300
Imaging Details
EM Software
TaskSoftware PackageVersion
MODEL FITTINGEMfit
RECONSTRUCTIONPFT
Image Processing
CTF Correction TypeCTF Correction DetailsNumber of Particles SelectedParticle Selection Details
CTF correction of each micrograph