1L5I

30-CONFORMER NMR ENSEMBLE OF THE N-TERMINAL, DNA-BINDING DOMAIN OF THE REPLICATION INITIATION PROTEIN FROM A GEMINIVIRUS (TOMATO YELLOW LEAF CURL VIRUS-SARDINIA)

SOLUTION NMR

NMR Experiment
Experiment	Type	Sample Contents	Solvent	Ionic Strength	pH	Pressure	Temperature (K)	Spectrometer
1	2D NOESY	0.8-1.0MM [10%-13C;U-99% 15N] REP4-121, SODIUM PHOSPHATE 20MM, NACL 100MM, DTT 1MM		0.3M	6.6	AMBIENT	298
2	3D_15N-SEPARATED_NOESY	0.8-1.0MM [10%-13C;U-99% 15N] REP4-121, SODIUM PHOSPHATE 20MM, NACL 100MM, DTT 1MM		0.3M	6.6	AMBIENT	298
3	HNHA	0.8-1.0MM [10%-13C;U-99% 15N] REP4-121, SODIUM PHOSPHATE 20MM, NACL 100MM, DTT 1MM		0.3M	6.6	AMBIENT	298
4	HNHB	0.8-1.0MM [10%-13C;U-99% 15N] REP4-121, SODIUM PHOSPHATE 20MM, NACL 100MM, DTT 1MM		0.3M	6.6	AMBIENT	298
5	4D_13C/15N-SEPARATED_NOESY	0.8-1.0MM [10%-13C;U-99% 15N] REP4-121, SODIUM PHOSPHATE 20MM, NACL 100MM, DTT 1MM		0.3M	6.6	AMBIENT	298
6	2D HNCOCG AROM	0.8-1.0MM [10%-13C;U-99% 15N] REP4-121, SODIUM PHOSPHATE 20MM, NACL 100MM, DTT 1MM		0.3M	6.6	AMBIENT	298
7	2D HNCO AROM	0.8-1.0MM [10%-13C;U-99% 15N] REP4-121, SODIUM PHOSPHATE 20MM, NACL 100MM, DTT 1MM		0.3M	6.6	AMBIENT	298
8	4D 13C,13C NOESY	0.8-1.0MM [10%-13C;U-99% 15N] REP4-121, SODIUM PHOSPHATE 20MM, NACL 100MM, DTT 1MM		0.3M	6.6	AMBIENT	298

NMR Spectrometer Information
Spectrometer	Manufacturer	Model	Field Strength
1	Bruker	DMX	500
2	Bruker	DMX	600
4	Bruker	DMX	750
5	Bruker	DRX	800

NMR Refinement
Method	Details	Software
TORSION ANGLE SIMULATED ANHEALING		NMRPipe

NMR Ensemble Information
Conformer Selection Criteria
Conformers Calculated Total Number
Conformers Submitted Total Number	30

Additional NMR Experimental Information
Details	THIS ENTRY CONTAINS THE 30-CONFORMER ENSEMBLE. THE MINIMIZED AVERAGE STRUCTURE OF THIS ENSEMBLE HAS BEEN DEPOSITED UNDER CODE 1L2M, AND IT IS A GOOD REPRESENTATION OF THE 30-CONFORMER ENSEMBLE CONTAINED HERE.

Computation: NMR Software
#	Classification	Version	Software Name	Author
1	processing	NMRPipe
2	data analysis	NMRView	4.1.1
3	data analysis	TALOS
4	structure solution	DYANA
5	refinement	DYANA

RCSB PDB Core Operations are funded by the National Science Foundation (DBI-1832184), the US Department of Energy (DE-SC0019749), and the National Cancer Institute, National Institute of Allergy and Infectious Diseases, and National Institute of General Medical Sciences of the National Institutes of Health under grant R01GM133198.