1L2M

Minimized Average Structure of the N-terminal, DNA-binding domain of the replication initiation protein from a geminivirus (Tomato yellow leaf curl virus-Sardinia)

SOLUTION NMR

NMR Experiment
Experiment	Type	Sample Contents	Solvent	Ionic Strength	pH	Pressure	Temperature (K)	Spectrometer
1	2D NOESY	0.8-1.0mM [10%-13C;U-99% 15N] rep4-121, sodium phosphate 20mM, NaCl 100mM, DTT 1mM	8%D2O,92%H2O	0.3M	6.6	ambient	298
2	2D NOESY	0.8-1.0mM [10%-13C;U-99% 15N] rep4-121, sodium phosphate 20mM, NaCl 100mM, DTT 1mM	100% D2O	0.3M	6.6	ambient	298
3	3D_15N-separated_NOESY	0.8-1.0mM [10%-13C;U-99% 15N] rep4-121, sodium phosphate 20mM, NaCl 100mM, DTT 1mM	8%D2O,92%H2O	0.3M	6.6	ambient	298
4	HNHA	0.8-1.0mM [10%-13C;U-99% 15N] rep4-121, sodium phosphate 20mM, NaCl 100mM, DTT 1mM	8%D2O,92%H2O	0.3M	6.6	ambient	298
5	HNHB	0.8-1.0mM [10%-13C;U-99% 15N] rep4-121, sodium phosphate 20mM, NaCl 100mM, DTT 1mM	8%D2O,92%H2O	0.3M	6.6	ambient	298
6	4D_13C/15N-separated_NOESY	0.8-1.0mM [U-13C;U-99% 15N] rep4-121, sodium phosphate 20mM, NaCl 100mM, DTT 1mM	8%D2O,92%H2O	0.3M	6.6	ambient	298
7	2D HNCOCG arom	0.8-1.0mM [10%-13C;U-99% 15N] rep4-121, sodium phosphate 20mM, NaCl 100mM, DTT 1mM	8%D2O,92%H2O	0.3M	6.6	ambient	298
8	2D HNCO arom	0.8-1.0mM [10%-13C;U-99% 15N] rep4-121, sodium phosphate 20mM, NaCl 100mM, DTT 1mM	8%D2O,92%H2O	0.3M	6.6	ambient	298
9	4D 13C,13C NOESY	0.8-1.0mM [10%-13C;U-99% 15N] rep4-121, sodium phosphate 20mM, NaCl 100mM, DTT 1mM	8%D2O,92%H2O	0.3M	6.6	ambient	298

NMR Spectrometer Information
Spectrometer	Manufacturer	Model	Field Strength
1	Bruker	DMX	500
2	Bruker	DMX	600
3	Bruker	DRX	600
4	Bruker	DMX	750
5	Bruker	DRX	800

NMR Refinement
Method	Details	Software
torsion angle simulated anhealing		NMRPipe

NMR Ensemble Information
Conformer Selection Criteria
Conformers Calculated Total Number
Conformers Submitted Total Number	1
Representative Model	(minimized average structure)

Additional NMR Experimental Information
Details	This entry contains the minimized average structure of the 30-conformer ensemble deposited under code 1L5I. The single model included in this entry is a good representation of the 30-conformer ensemble.

Computation: NMR Software
#	Classification	Version	Software Name	Author
1	processing	NMRPipe
2	data analysis	NMRView	4.1.1
3	data analysis	TALOS
4	structure solution	DYANA
5	refinement	DYANA

RCSB PDB Core Operations are funded by the National Science Foundation (DBI-1832184), the US Department of Energy (DE-SC0019749), and the National Cancer Institute, National Institute of Allergy and Infectious Diseases, and National Institute of General Medical Sciences of the National Institutes of Health under grant R01GM133198.